Scientists ‘break the mould’ by creating new colours of ‘blue cheese’

Spin-off will examine if the new strains can be commercialised

13-Feb-2024
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Experts at the University of Nottingham have discovered how to create different colours of blue cheese. After discovering how the classic blue-green veining is created, a team of experts from the School of Life Sciences, were able to create a variety of different fungal strains that could be used to make cheese with colours ranging from white to yellow-green to red-brown-pink and light and dark blues. The findings of the study are published in the journal NPJ Science of Food.

The University of Nottingham

Left hand side. Spectrum of colour strains produced in Pencillium roqueforti (wild type to far right). Right hand side. Cross sections of cheeses made with the original (dark blue-green) or new colour (red-brow, bright green, white albino) strains of the fungus.

The fungus Penicillium roqueforti is used worldwide in the production of blue-veined cheese such as Stilton, Roquefort and Gorgonzola. Its unique blue-green colour and flavour comes from pigmented spores formed by fungal growth. Using a combination of bioinformatics, targeted gene deletions and heterologous gene expression, the research team, led by Dr Paul Dyer, Professor of Fungal Biology, learnt the way in which the blue-green pigment is produced.

The researchers found that a biochemical pathway gradually forms the blue pigments, starting at a white colour, which progressively becomes yellow-green, red-brown-pink, dark brown, light blue, and finally dark blue-green. The team were then able to use some classic food safe (non GM) techniques to ‘block’ the pathway at certain points, and so create strains with new colours that can be used in cheese production.

Dr Dyer said: “We’ve been interested in cheese fungi for over 10 years and traditionally when you develop mould-ripened cheeses, you get blue cheeses such as Stilton, Roquefort and Gorgonzola which use fixed strains of fungi that are blue-green in colour. We wanted to see if we could develop new strains with new flavours and appearances.

“The way we went about that was to induce sexual reproduction in the fungus, so for the first time we were able to generate a wide range of strains which had novel flavours including attractive new mild and intense tastes. We then made new colour versions of some of these novel strains."

Once the team produced the cheese with the new colour strains, they then used lab diagnostic instruments to see what the flavour might be like.

“We found that the taste was very similar to the original blue strains from which they were derived,” said Dr Dyer. “There were subtle differences but not very much.

“The interesting part was that once we went on to make some cheese, we then did some taste trials with volunteers from across the wider University, and we found that when people were trying the lighter coloured strains they thought they tasted more mild. Whereas they thought the darker strain had a more intense flavour.  Similarly, with the more reddish brown and a light green one, people thought they had a fruity tangy element to them – whereas according to the lab instruments they were very similar in flavour. This shows that people do perceive taste not only from what they taste but also by what they see.”

The team, which included lead postgraduate student Matt Cleere, will now look at working with cheese makers in both Nottinghamshire and Scotland to create the new colour variants of blue cheese. A University spin-out company called Myconeos has also already been established to see if the strains can be commercialised.

“Personally, I think it will give people a really satisfying sensorial feeling eating these new cheeses and hopefully might attract some new people into the market,” adds Dr Dyer.

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